Mass screening method of rice [Oryza sativa] cultivars and breeding lines for brown planthopper, Nilaparvata lugens Stal
1997
Saka, N. (Aichi-ken. Agricultural Research Center, Nagakute (Japan)) | Tsuji, T. | Izawa, T.
In order to breed japonica rice cultivars resistant to the brown planthopper, a method of mass screening in green houses was developed. The results are summarized as follows: 1) Mass rearing of BPH was possible by using 8 rearing trays of 19 x 13 x 4 (height) cm with rice seedlings at about the third leaf stage, in a case of 56 x 32 x 50 (height) cm. The mass rearing for screening was started in 2 trays with 800 BPH enclosing 6 trays with fresh rice seedlings in 1 cage. After 3 weeks we acquired the 15000 BPH for mass screening in1 cage. Two check varieties were sown in the nursery bed at the start of mass rearing for screening. Testing was done 3 weeks after the time of sowing. 2) The results of BPH mass screening in the green house were affected by inoculation leaf stage, the number of BPHs and the application of fertilizer to test seedlings. It was proved that the most exact difference between resistant and susceptible conditions of screening was at about the third leaf stage of the test seedlings, with no fertilizer application. 3) Due to the mass \screening, we were able to adopt a hybrid population. From these results, a manual was prepared for the mass screening of rice cultivars and lines for BPH
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