Measurement of undegradable grass hay ribonucleic acid by in sacco and in vivo methods and their effect on the estimation of microbial production in the rumen of sheep
1998
Zhao, G. (Iwate Univ., Morioka (Japan). Faculty of Agriculture) | Sasaki, H. | Kurosawa, M. | Sakai, M. | Nakashima, Y.
Four sheep, fitted with ruminal and duodenal cannulas and given only orchardgrass hay, were used for in sacco and in vivo estimation of the ruminal degradation of the hay ribonucleic acid (RNA) and for measurement of duodenal digesta flow. Other four rumen cannulated sheep given orchardgrass hay wee used to isolate bacterial and protozoal samples from strained rumen content and to determine the concentration of RNA (purines), diaminopimelic acid (DAPA) and phosphatidylcholine (PC) as markers of rumen microbes. Samples of hay and duodenal digesta were also analyzed for these markers. The degradation characteristics in sacco of hay RNA, dry matter (DM) and crude protein (CP) were measured by incubating hay sample in polyester bags in the rumen
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