Cell wall architecture prerequisite for the cell division in the protoplasts of white poplar, Populus alba L
Suzuki, K. (Kyoto Univ., Uji (Japan). Wood Research Inst.) | Itoh, T. | Sasamoto, H.
White poplar (Populus alba L.) protoplasts were investigated at 0, 3, 10, 20 and 30 d after regeneration to visualize the cell wall architecture prerequisite for cell division. The 10 day-old cells just before cell division developed a thin wall layer with uneven deposition of cell wall materials and were saved from bursting by suspension in low osmotic medium. The three dimensional architecture of the cell wall, as revealed by rapid-freezing and deep-etching electron microscopy, in 10 day-old cells, constituted thin inner most lamellae on the plasma membrane along with highly extended microfibrillar networks. These results suggest that the deposition of thin lamellae is important not only for cells to withstand bursting but also to induce cell division. The present investigations give the first account of the visualization of the three-dimensional architecture of regenerated cell wall right before cell division
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