Basic studies on artificial insemination of ducks
1997
Kasai, K. (Osaka-fu. Agricultural and Forestry Research Center, Habikino (Japan)) | Izumo, A.
1. Receptacles for semen collection: Glass funnels and small plastic cups were tested as the receptacles for semen collection. Glass funnels could be applied to all drakes but the amount of collected semen was significantly smaller than that of semen collected with plastic cup. Plastic cups could be adopted only to exceptional drakes those ejaculate without evaginating penis, but the much more amount of semen could be obtained with less contamination. 2. Semen characteristics: Characteristics of drake semen were examined. The results from 129 samples showed that the average volume of collected semen was 0.29 ml per ejaculate, containing 1.28 billions sperms per ml. The average number of sperms per ejaculate was 408 millions and the average pH-value of semen was 7.29. 3. Semen diluents: The effects of 4 kinds of diluents on fertilizing capacity of duck sperm in immediate insemination and on sperm motility in the semen stored at 4 degrees C were examined. The mean fertility during the period from 2 to 8 days following use of semen diluted with Lake's solution, phosphate buffer, PBS (+), physiological saline and undiluted (control) was 72.3, 73.3, 64.4, 69.1 and 53.9%, respectively. High sperm motility was maintained for 8 hours in the semen diluted with Lake's solution and phosphate buffer. But in the semen diluted with PBS (+) and physiological saline, the percentage of the sperms with bendin mid-piece was increased after 4 hours of storage. 4. Optimum sperm number for a single insemination: Fertility tests were carried out with pooled semen contains 5 different number of sperms. The mean fertility during the period from 2 to 8 days after a single insemination of each semen was 76.9, 77.8, 59.7, 59.4 and 29.9%, respectively
Показать больше [+] Меньше [-]