Effect of corticosterone on growth and proteolysis in primary cultured chick muscle cells
1998
Nakashima, K. (Kagoshima Univ. (Japan). Faculty of Agriculture) | Nakamura, S. | Ohtsuka, A. | Hayashi, K.
The effect of corticosterone (CTC) on growth and proteolysis in primary cultured chick muscle cells was studied. The muscle cells were incubated with physiological level of CTC (0.3 mu-g/100 ml) for 6 days. The media were replaced every two days. Protein and DNA contents were measured as indicates of cell growth and cell proliferation, respectively, after the cells were harvested on the final day of the incubation. Creatine kinase (CK) activity, myosin content and actin content were also measured as indices of myotube formation. The medium was collected to measure Ntau-Methylhistidine concentration as an index of myofibrillar proteolysis. Protein content and DNA content increased during incubation, while CK activity and myosin and actin contents decreased. Ntau-Methylhistidine release was not affected by CTC treatment. These results indicate that cell proliferation but not myotube formation and proteolysis are stimulated by CTC in primary chick muscle cell culture
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