Progressive effects of phloridzin on melanogenesis in B16 mouse melanoma cells
Shoji, T. (Nikka Whisky Distilling Co. Ltd., Kashiwa, Chiba (Japan)) | Kobori, M. | SHinmoto, H. | Tanabe, M. | Tsushida, T.
When we studied the effects of polyphenols from apple fruits on melanogenesis in B16 mouse melanoma cell lines, phloridzin had dose-dependent progressive effects on melanogenesis between 10 and 500 mu-g/ml without inhibiting cell growth. At a concentration of 500 mu-g/ml, phloridzin increased the melanin content in the cells to 8% of that in control cells. In contrast, phloretin, the aglycon of phloridzin, aid activate melanogenesis in the cells and was cytotoxic at a concentration of 5 mu-g/ml. Phloridzin increased the activity of tyrosinase to 223% of that in control cells. Furthermore, phloridzin inhibited the activity of protein kinase C (PKC), which is recognized to regulate tyrosinase activity. The inhibition of PKC activity continued for 12 min from the addition of phloridzin. Therefore, we estimated that the activation of melanogenesis by phloridzin resulted from the increase of tyrosinase activity caused by the inhibition of PKC activity
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