Ioslation and Identification of Barley Yellow Mosaic Virus in Korea
1998
Lee Kui Jae | So In Young College of Agricultural Biology, Chonbuk Nat'l Univ., Chonju 561-756 (Korea Republic)
Barley yellow mosaic virus (BaYMV-HN) occurring Haenam area was isoltaed by mechanical inoculation onto barley cultivars, purification and production of antibody. BaYMV-HN were purified from infected plants a filamentous virus with 13 nm in diameter and 250~300 nm and 500~650 nm in length. Specific antibody made by injecting the purified virus to the muscle of a rabbit. In gel-diffusion tests antibody to BaYMV-HN did not make spur with two Japanese BaRMV-HN isolates, BaYMV-2-1 or BaYMV-3. BaYMV-HN showed the symptom of yellowing and necrosis in host plants. Mechanical inoculation tests with Japanese barley cultivars showed that BaYMV-HN infected New Golden, Akagi Nijo and Tosan Kawa 73, but did not infect Amagi Nijo, Haruna Nijo, Ishukushirazu (ym3), Misato Golden (Ym1), Kashimamugi, Joshushiro Hadaka and Mokusekko 3 (ym1). In Korean barley cultivars, some of the naked barleys which are Hinssalbori, Kinssalbori, Saessalbori and Saechalssalbori were not infected by BaYMV-HN. However, it ingected all the covered barley cultivars and the beer barley cultivars. BaYMV-HN had two RNAs, RNA 1 (7.6 Kb) and RNA 2 (3.5 Kb), and one coat protein (33 KDa).
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