Role of polycation for enhancing infectivity of retrovirus
1999
Kang, S.H. | Kim, S.C. | Lee, S.G. | Kim, B.G. (Seoul National University, Seoul (Korea Republic). Institute of Molecular Biology & Genetics and School of Chemical Engineering)
To verify ebhancing effect of polycation on the retroviral infectivity, we directly measured the binding affinity of retroviruses to the target cells in the presence or in the bsence fo polybrene wkth R18 fluorescence assay and examined the effect of the polymers on the relationship between the host cell and the retroviral infectivity. There was no difference in the effect of the types of charge of the polymers on the binding affinity. However, Polycations, inn general, show effect on the retrovirus infectivity. Theis results suggest that the enhancing effect of polybrene and other polycations on the infectivity is not due to the binding step but due to the post-binding steps, especially the internalization step. With the result of the internalization of FITC-labeled poly-L-lysine into the host cells, it is suggested that the uptake of polycations into the host cells would play a crucial role in the internalization of retroviruses.
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