Effect of phosphorylated oligosaccharide from potato-starch on calcium absorption in rats
1998
Kishimoto, M. (Mukogawa Women's Univ., Nishinomiya, Hyogo (Japan)) | Kamasaka, H. | Murakami, A. | Kawaguchi, M. | Matsuura, T. | Okada, S. | Ichikawa, T.
Previously, we observed the inhibitory effect of phosphorylated oligosaccharides (PO) from potato-starch on the formation of calcium phosphate precipitate. PO were composed of two fractions: PO-1 and PO-2. The former was malto oligosaccharide bound to one phosphate molecule, and the latter was malto oligosaccharide bound to at least two phosphate molecules. PO-2 had a stronger inhibitory effect. In the present study, we investigated the effect of PO-2 feeding on calcium utilization in rats. Five-week-old rats were fed a calcium diet (0.52% of calcium) containing 5% PO-2 for two weeks. The apparent calcium absorption ratio and apparent retention ratio during two periods (day 3-6, days 10-14) were significantly lower in the rats fed the PO-2 diet than in those fed the control diet. The results of a breaking-force test and the calcium content of the femur did not differ significantly between the two groups. Whole cecal weight was significantly higher in the rats fed the PO-2 diet, and the amounts of short-chain fatty acid in the cecal content were significantly increased; cecal pH was not changed. The was no apparent correlation between the calcium solubilizing effect of PO-2 in vitro and the degree of calcium absorption in vivo
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