Preculturing conditions for cryopreservation of conchocelis of Porphyra yezoensis form. narawaensis
1997
Fujiyoshi, E. (Seikai National Fisheries Research Inst., Nagasaki (Japan)) | Umezawa, S.
Various preculturing conditions were studied in order to improve the survival rates of conchocelis cells of Porphyra yezoensis form. narawaensis after freeze-thaw cycle. The preculturing conditions tested were combinations of temperature (6 or 18 degrees C), period (1 or 3 days) and with or without cryoprotectant (0.5 M sorbitol, 0.9M sorbitol or 0.5M DMSO). The precultured cells were then treated with the cryoprotective solution containing 1.5M DMSO and 0.5M sorbitol, cooled at 1 degrees C/min to -45 degrees C and plunged into liquid nitrogen. After the freeze-thaw cycle, the survival rates of the precultured cells were determined. It was found that preculturing cells with 0.5M sorbitol, 0.9M sorbitol or 0.5M DMSO at 5 degrees C for 3 days and with 0.5M sorbitol at 18 degrees C for 1 day improved the survival rate as much as 20 compared with the cells precultured without cryoprotectant at 18 degrees C
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