Changes in catechin and procyanidin contents during fruit development of two apple cultivars
1999
Hamauzu, Y. (Shinshu Univ., Minamiminowa, Nagano (Japan). Faculty of Agriculture) | Iijima, E. | Banno, K.
Catechins, procyanidins and other major phenolics in developing Tsugaru and Fuji apples fruit were identified and quantified by spectrophotometric, thin layer chromatographic and high performance liquid chromatographic assays and their changes observed. 1) Polymeric procyanidin was the most abundant polyphenol in both cultivars. Chlorogenic acid, other hhydroxycinnamic esters, (+)-catechin (-)-epicatechin, oligomeric procyanidins and phloridzin were major phenolics in the ethyl acetate extractable fraction. 2) The total amount of catechins and procyanidins (total catechin units) content decreased on a fresh weight basis, whereas it increased on a fruit basis during fruit development in both cultivars. 3) Catechins (monomeric form of procyanidins) and polymeric procyanidin were the predominant components in flavan-3-ol group 30 days after pollination (DAP). Oligomeric procyanidins and (-)-epicatechin content on a fruit basis increased by the middle stage of development and decreased thereafter, whereas polymeric procyanidin increased as fruits matured. 4) (+)-Catechin, procyanidin B1 and especially chlorogenic acid content in fuji were higher than those in Tsugaru. Chlorogenic acid content increased only in Fuji. Although phenolic contents in Tsugaru and Fuji were slightly different, the pattern of seasonal changes in catechins and procyanidins contents was similar. Apparently, monomeric and oligomeric procyanidins which temporarily increased were concerted to polymeric
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