Effect of hormonal and nutritional factors on morphogenesis of Pinus sylvestris L. in vitro
2000
Andersone, U. | Ievins, G. (University of Latvia, Riga (Latvia). Inst. of Biology)
The morphogenesis of Pinus sylvestris L. in vitro was investigated. Pine seedlings grown on medium containing 50% Murashige-Skoog mineral salts plus 50% Bourgin-Nitsch organic compounds plus sucrose without hormones formed shoots with needles. Transfer of hypocotyl cuttings to cytokinine-containing medium induced microshoot development. An inverse relationship between the number of newly formed microshoots and the length of the hypocotyl shoots before transplantation was established. Growth of buds was stimulated by transfer to a hormone-free medium. On shoot explants microshoot density was related to microshoot length. For initiation of elongation of newly formed microshoots, explants were tranferred to a hormone-free medium with diminished amounts of minerals and supplemented with activated charcoal.
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