Selection of effective cowpea rhizobial strains for Centrosema pubescens
1994
Sumittra Puvarodom (King Mongkut's Institute of Technology Ladkrabang, Bangkok (Thailand). Faculty of Agricultural Technology)
Selection of effective nitrogen fixation rhizobial strains for Centrosema pubescens was carried out in three separate experiments. The first experiment was conducted to compare the two growth system for counting population of soils by whole soil technique. The result indicated that Centrosema grown in Niftal tube results in faster nodule formation (14 days) than Gibson tube which required 23 days for nodule formation. When soil rhizobial number was lower than 10*[2) cells/g of soil, plant grown in Gibson tube failed to nodulate whereas in Niftal tube the nodule was found. This experiment indicated that Niftal tube can be used for counting rhizobium in soil by plant infection test. Enumeration of indigenous soil rhizobial population for Centrosema was conducted in 34 soil samples. Nodules were found in 23 soil samples. The highest rhizobia estimate was 7.34x10*[5) cells/g soil. It was also found that soil with rhizobia number higher than 10*[3) cells/g soil were collected from the area where Centrosema were grown at the time of sampling. Soil samples collected from plots growing other crops even it was closed the where Centrosema was grown, the population of rhizobia was low or not found. The third experiment, the result indicated that plant inoculated with 10*[-1) dilution grown better than 10*[-3) dilution. Soils with rhizobia number more than 10*[5) cells/g soil resulted in higher shoot and root dry matter weight and nodule number compared to soil with rhizobia number. The rhizobium in these soils were found to be more effective than Rhizobium strain TAL 655 but not as effective as strain TAL 652.
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