Biotechnology and biochemical analysis of root exudates of maize (Zea mays) grown in acidic pH media
1997
Poontariga Harinasut | Nitsri Sangduen | Chamnan Chutkaew (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biochemistry)
High oil maize (KOSX 3503) grown well in the acidic pH media (Harinasut, P. et al., Full research report, KURDI, 1995) was used in the study on secretion from maize root. The present research was studied on glycoproteins which were secreted in liquid medium by suspension-cultured cells developed from roots of maize seedlings. Calli were developed from root of maize seedlings and suspension-cultured cells were developed from calli. The suspension-cultured cells grown in the suitable liquid-cultured medium could get log phase within 5 days. The liquid media of 7 day-old suspension-cultured cells grown in the media of pH 5.0 and 6.5 were compared with the extract from the calli. Proteins were precipitated with 85 percent ammonium sulfate saturation. After dialysis, proteins were separated by electrophoresis technique. The detection of glycoprotein containing glucose-manose was carried out by affinoblotting technique. The glycoprotein secreted in both liquid media showed the same pattern as those from the extract of calli. The major glycoproteins were 66, 52, 46, 43, 41, 32 and 21 kilodalton. The 43 and 41 kilodalton glycoproteins were strongly secreted in the liquid media. The 46 kilodalton glycoprotein was secreted in the liquid medium but absent from the extract of calli.
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Эту запись предоставил Kasetsart University