Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta)
2001
Dvoracek, V. | Curn, V. | Moudry, J. (Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta)
Esterase, aspartate aminotransrerase, glutamate dehydrogenase, malate dehydrogenase, shikimate dehydrogenase isozymes, protein extracted 2% glutathion buffer (separated from leaves and seed in non-denaturing PAGE system) and seed proteins separated in SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat varieties (Hercule, Rouquin, Altgold), three breeders` spelt lines (H29.27, H92.28, M92.20 originated from hybridisation between spelt and common wheat) and reference common wheat variety (Brea). Only evaluation of three marker systems showed differences among common wheat variety Brea and spelt varieties and spelt breeders' lines.
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