Changes of isoflavonoid levels in pea (Pisum sativum) seeds, seedlings and plants influenced by UV-A and gamma-irradiation
2001
Lachman, J. | Orsak, M. | Lapcik, O. | Pivec, V. (Ceska Zemedelska Univ., Prague-Suchdol (Czech Republic)) | Hosnedl, V.
In two pea cultivars (Lantra, Menhir), changes in isoflavonoid levels caused by UV-A and by irradiation were investigated. The changes were determined in seeds, seedlings and plants. UV-A irradiation was performed for 0 (control), 24, 48 and 72 hours at lambda = 365.5 nm, P = 125 W, gamma-irradiation with **60 Co at doses 0 (control), 10, 20 and 40 Gy. Cultivars responded differentially to various doses of irradiation in both types of irradiation. Daidzein (Dai) and genistein (Gen) were determined by a selective and sensitive radioimmunoassay 7- and 4'-cross-reactive method, biochanin A (Bio A) was determi-ned by means of 7-cross-reactivity. The highest average levels among isoflavonoids were found for daidzein in both pea varieties (6 562.4 microg/kg DM in Lantra seeds, 3,481.3 microg/kg DM in Mantra seeds). The isoflavonoid content signiflcantly increased during sprouting of seeds and plant growth. UV-A irradiation caused apparent increase of isoflavonoids at high levels (72 hours) of irradiation, especially in seedlings and plants. The isoflavonoid increase was higher in seedlings compared with plants. Low doses of UV-A irradiation had little effect on the increase of isoflavonoids, while gamma-irradiation was the most efficient at low doses of 10 Gy and 20 Gy. Seedlings and plants differed in the reaction to irradiations.
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