Studies on Effect and its Mechanism of Herbicide Mixture of Cyhalofop-bytyl, pyribenzoxim and Pyrazosulfuron-ethyl 2. Effect of Herbicide Mixture on the Activity of ALS, Content of Free Amino Acids and Fatty Acids
1998
Wu Ming-Gen | Kim Kil-Ung Department of Agronomy, College of Agriculture, Kyungpook National University, Taegu 702-701 (Korea Republic)
This study was conducted to determine the activity of ALS, content of endogenous free amino acids and fatty acids affected by herbicide mixture of cyhalofop-butyl, pyribenzoxim and pyrazosulfuron-ethyl. I50 values (concentration required for 50% inhibition of ALS activity) of pyribenzoxim herbicide on the activity of ALS in Echinochloa crus-galli and Cyperus serotinus in vitro were recorded at 4*100 nM and 5*10 nM, respectively, while I50 values of pyrazosulfuron against E. crus-galli and C. serotinus were 4.5*10 nM and 4*10 nM, respectively, and the mixture of two herbicides showed additive effect on ALS activity at the low application rate, and independent effect at the high application rates of two herbicides. The inhibition rates of the three herbicides mixture treatment on the three branch-chain amino acids such as valine, leucine and isoleucine were 74.6%, 66.6% and 57.9% in C. serotinus and 36.6%, 51.1% and 48.1% in E. crus-galli, respectively. A little bit higher inhibitory effect on the three branch-chain amino acids in C. serotinus and E. crus-galli seedlings was observed in two herbicide mixture of pyribenzoxim with pyrazosulfuron than three herbicide mixture of cyhalofop with pyribenzoxim and pyrazosulfuron. The interaction among three herbicides showed non-antagonism on the amounts of endogenous free amino acids.
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