Qualitative and quantitative estimation on indole-3-acetic acid (IAA) in the seeds of Shorea robusta
1999
Bhattacharya, A. | Das, B. | Saha, P.K. (Bose Institute, 93/1, Acharya Prafulla Chandra Road, Calcutta 700009 (India). Dept. of Botany)
Qualitative and quantitative estimation of indole-3-acetic acid (IAA) in the seeds of Shorea robusta. In India, Shorea robusta (Family:Dipterocarpaceae) is widely distributed forming extensive forests. This economically important tree could not be exploited by foresters due to the short viability period of the seeds. Among the several factors responsible for early induction of non-viability, indole acetic acid (IAA) is one which varied both qualitatively and quantitatively in the seeds at different stages of maturation. It was observed that the amount of IAA in the seeds decreased with maturity. In immature seeds, the content of free and bound IAA were 0.00750 miu gram per gram and 0.00146 miu gram per gram of dry seeds respectively. In mature viable seeds both free and bound IAA levels declined to 0.00132 miu gram per gram and 0.00063 miu gram per gram of seed respectively. The non-viable seeds showed bound IAA of 0.0012 miu gram per gram of seed but no free IAA. In the wheat coleoptile extension bioassay, considerable growth promoting activity was observed in the case of free IAA but no significant activity was recorded in the case of bound IAA.
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