Enzymatic production of hydroxamic fatty acids from coconut oil by a lipase from Rhizopus delemar
2000
Fundador, N.G.V.
A crude lipase for Rhizopus delemar was used to catalyze the production of Hydroxamic Fatty Acids (HFA) by reacting hydroxylamine - HCl with coconut oil, methyl oil, methyl laurate and lauric acid. The various reaction parameters such as amount of hydroxylamine - HCl, incubation time, temperature and amount of enzyme were evaluated with the aim of achieving high yield of HFA. The reaction products were monitored by Thin Layer Chromatography (TLC) using hexane: diethyl ether: acetic acid (20:80:1 v/v/v) as solvent system and quantified by Gas-Liquid Chromatography (GLC) equipped with Flame Ionization Detector (FID). The common type of HFA produced by the three substrates was laurylhydroxamic acid (LHA). The percentage compositon of LHA was observed to be lower (45.50%) using coconut oil as substrate compared to methyl laurate (81.94%) and lauric acid (83.82%). This could attribute to the presence of other types of HFA formed from coconut oil namely myristylhydroxamic acid (30.33%) and dolyhydroxamic acid (24.07%); hence, affecting the percent composition of LHA. The yield of HFA depended strongly on the reaction temperature regardless of the substrate used. Purification of the product was done by crystallization in hexane at 14 deg C. The purity and identification of the functional groups present were determined by IR spectroscopy
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