Development of immunity to coccidiosis in chicken administrated sonicated coccidial vaccine
Akhtar, M. | Hayat, C.S. | Ayaz, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Parasitology) | Ashfaque, M. | Ayaz, M.M. | Hussain, I.
Humoral immune response of Vaccine-I (supermatant from sonicated sporulated oocyst), Vaccine-II (sediment from sonicated sporulated oocyst) and Vaccine-Ill (un-sonicated sporulated oocyst) against coccidiosis in chickens was determined by indirect haemagglutination (IHA) test. IHA antibody titre was significantly higher (P0.05) in chicks vaccinated with Vaccine-I as compare to Vaccine-11 and Vaccine- Ill. The IHA antibody titre of chicks vaccinated with Vaccine-I ranged from 1:8 to 1:8192, 1:16 1:512 for Vaccine II and 1:2 to 1:64 for Vaccine III. Vaccine I gave 100 per cent protection and oocysts appeared in the faeces (100-200 per gram) on day 10 post challenge which gradually increased (600-900 per gram) on day 16 and 200-400 on day 20 post challenge. Vaccine II gave 60 per cent protection and oocyst appeared in the faeces (3,50,000-4,50,000 per gram) on day 8 post challenge which gradually increased (700,000-900,000 per gram) on day. 16 and 100, 000- 200,000 on day 20 post challenge. Vaccine III gave 30 per cent protection and oocyst appeared in the faeces (8,50,000-9,00,000 per gram) on day 7 post challenge which gradually increased (11,400,000-12,00,000 per gram) on day 16 and 8,40,000-9,00,000. on day 20 post challenge. In control group, characteristic bloody diarrhoea was observed in all the chicks and oocysts appeared in the faeces (10,00,000-12,50,000 per gram) on day 5 post challenge of faeces which gradually increased (15,00,000-17,50,000 per gram) on day 16 post- challenge. Results of the humoral and challenge response indicated that the Vaccine-I induced a strong protection as immune chicks contained high level of antibodies that resisted heavy dose of challenge and gave 100 per cent protection.
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