Genetic diversity in the B genome of Musa germplasm assessed by RAPD [random amplified polymorphic DNA] technique
2002
Sales, E.K. (University of Southern Minadanao, Kabacan, 9407 Cotabato (Philippines) Dept. of Plant Breeding and Genetics (Espino, R.R.C. | Barrion, A.A. | Mendioro, M.S. | Cedo, M.L.O.
The genetic diversities and relationships among 19 Philippine Musa cultivars based on the DNA fingerprints were investigated. DNA of the 19 cultivars extracted through modified CTAB protocol were analyzed using random amplified polymorphic DNA(RAPD) technique. A total of 118 random primers were used in the analysis. Of these primers, 41 were identified useful in distinguishing the 19 Musa cultivars. Primers J-04, S-02, and AJ-01 clearly showed genetic divergence in the 19 cultivars as evidenced by the different band, detected three DNA markers, which are present in all the BB/BBB cultivars. Through these DNA markers (0.560 kb marker detected by primer OPH-03 and 0.836 kb markers detected by Primer OP1-16 the genomic sequence constitution of six Musa collections was determined. Cluster analysis at specific similarity coefficients revealed that the 11 M. balkesiana cultivars evaluated are different and distinct from M. acuminata cultivars as represented by 'Bungulan' and 'Lakatan'. It also suggests that the three Pinatubo collections are of the BB/BBB genome while the three Palawan collections might be of the AA/AAA type. Some degrees of variability were also detected among the BB/BBB cultivars. This study demonstrated that RAPD could be used for Musa germplasm characterization, identification and fingerprinting. It is useful in establishing phenetic and phytogenetic relationships as well as genomic constitution of Musa cultivars. It can also be used in detecting genetic diversities particularly in the B genome groups of Musa cultivars
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