Potential use of the biotech produced beta-monoglyceride as protectant of tomato, cabbage and Chinese cabbage against soft rot disease (Erwinia carotovora pv. carotovora)
Espino, T.M. | Abad, J.D. | Reyes, J.G. | Tambalo, F.Z. | Tambalo, R.D. (Philippines Univ. Los Banos, College, Laguna (Philippines). National Inst. of Molecular Biology and Biotechnology
This study was conducted to determine the potential use of the BIOTECH-produced beta-monoglyceride (B-MG) as protectant to tomato, cabbage, and Chinese cabbage against soft rot disease caused by Erwinia carotovora pv. carotovora. The pathogenic bacterium was used to evaluate the antimicrobial activity of B-MG in vitro using the broth dilution susceptibility test. Results showed that the minimum lethal concentration (MLC) of the bacterium was 10 ppm and the minimum inhibitory concentration (MIC) was between five to ten ppm. The evaluation on the efficacy of B-MG as protectant of fruits and vegetables in vivo against the pathogen was done to further substantiate the findings. Results showed that B-MG at 10 and 50 ppm did not inhibit bacterial infection on all the crops tested namely, tomato, cabbage and Chinese cabbage but 100 ppm totally prevented the infection caused by the soft rot bacteria on all the crops tested. Thus, 100 ppm B-MG is the most effective concentration that could be used as protectant to fruits and vegetables against the pathogen. No undesirable changes in color, firmness and odor were observed in all the test samples coated with B-MG compared with the uninoculated control. Hence, B-MG has no effect on the aesthetic quality of the samples tested
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