Tissue culture of Broussonetia luzonica (Blanco) Bur. var. luzonica (Himbabao, Family Moraceae)
2002
Ungson, L.B. (Philippines Univ. Diliman, Diliman, Quezon City (Philippines). Coll. of Science) Lacuarta, G.G.
Broussonetia luzonica (Blanco) Bur. var. luzonica (Himbabao, Family Moraceae) is a Philippine endemic forest tree whose current conservation status is that of depleted ecologically in the wild. It bears edible staminate flowers consumed as vegetable by village residents wherever it occurs. Tissue culture as an alternative propagation technique of this plant was studied. Young leaves from shoots of rooted large diameter-stems were the source of explants. For callus induction, sterilized explants were grown in agar-solidified Murashige & Skoog (MS) media with 2% sucrose and 0, 1.0 and 10 microM each of 2,4-D in BAP [benzylaminopurine] in factorial combinations. Shoot development was continued in MS plus 10 microM BAP with and without 10 microM 2,4-D. Shoots were induced in media with 0.5 or 1.0 microM BAP. Shoots were rooted in MS media with 2 mg/l NAA and in 1 mg/l each of NAA and IBA. Calli started to form 2-3 days after inoculation and continued proliferating with continuous subculture every 10-14 days. These formed initially along margins and veins of explants. The higest frequency of callus formation (100%) was in media with 10 microM each of 2,4-D and BAP. On the other hand, shoot elongation and development occurred 20-28 days after inoculation. Shoot buds were formed in media with only 10 microM BAP and in 10 microM each of BAP and 2,4-D. More shoots elongated in media with 0.5 or 1.0 microM BAP compared with 10 microM BAP. Elongated shoots rooted in MS medium with only 2 mg/l NAA and in 1 mg/l each of NAA and IBA. This study suggests feasibility of applying the "indirect" organogenesis method for propagation of this particular forest species and conservation
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