Characterization of cassava root of proteins albumin and globulin
1998
Jariya Sukhapan | Montri Chulavatnatol (Mahidol Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biochemistry)
Except for the cereals, tropical tuber crops are the most important group of staple food in the tropical countries. Tubers are generally rich in starch and poor in protein. Very little is known about these tubers proteins. In contrast, seed storage proteins have been extensively studied. The most studied tuber protein is patatin, a protein from potato tuber. This protein has been suggested to have a lipid acyl hydrolase activity and might have a function in protection against microbial growth. In sweet potato, sporamin has also suggested to be involved in the defense reaction against injury or fungal infection. To increase our knowledge of tuber proteins, we have studied two major proteins of cassava roots [Manihot esculenta crantz], albumin from the cortex and globulin from the parenchyma. By using Ammonium sulfate fractionation and gel filtration chromatography, albumin and globulin was separated. By the SDS-PAGE, Subunit molecular weight of cassava albumin was found to be 67,000 and that globulin was 22,000.
Показать больше [+] Меньше [-]