Studies on structure and function of sucrose phosphate synthase gene from rice (Oryza sativa L.)
1997
Duangjai Techayingpaiboon (University of Tsukuba (Japan))
Two cDNAs encoding sucrose phosphate synthase from rice plant (Orysa sativa L. Nipponbare) in the previous experiment were isolated. Both cDNAs, namely SPS1 and SPS2 cDNAs were determined to be 3231 bp and 2912 bp in length, respectively. This was the first report suggesting isozyme of rice sucrose phosphate synthase. The deduced amino acid sequence of both cDNAs was compared to those of maize cDNA and found that the homology was about 52 and 62 percent. respectively. However, the clones of the above SPS1 and SPS2 cDNAs did not appear to have complete sequence of rice sucrose phosphate synthase cDNA. There are some deletion parts in both SPS1 and SPS2 cDNAs and the 5' terminal sequences of both cDNAs have not been isolated yet. A preliminary experiment, the presence of deletion parts of both cDNAs were studies based on the data from GANETYX-MAC programming software. The deduced amino acid sequences of both cDNAs were compared to sucrose phosphate synthase cDNAs from different species and found that the deletion part of SPS1 cDNA occur around 230 and SPS2 occur around 573 of their amino acid sequence from N terminus. In the present study, identification of deletion part in the above clones and determination of the sequence of the deletion parts were studied. Also, isolation and sequence of genomic clones of SPS1 and SPS2 were examined in order to determine complete sequence of SPS1 and SPS2 cDNAs.
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