Detection of Glu D1 5+10 locus in donors of wheat genes (Triticum aestivum) with non-standard spike morphotypes
2003
Bednar, J. | Jedlickova, D. | Vinterova, M. | Vyhnanek, T. | Hurta, P. (Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Botaniky a Fyziologie Rostlin) | Martinek, P.
In twenty one-five selected gene resources of Triticum aestivum with the non-standard spike morphotype MRS (multirow spike) and LG (long glumes), the presence of Glu D1 5+10 glutenin subunits was tested using DNA markers. The presence of these loci is a genetic marker of high baking quality. The DNA for analysis was isolated from young wheat leaves using the DNAsy Plant Mini Kit. Specific sequences of nucleotides were determined by the Specific Polymorphic Locus Amplification Test (SPLAT), using primers JEDL 11 and JEDL 12. The allele Glu D1 5+10 was verified in all the MRS lines and in all LG lines. These genotypes are used for further crosses and breeding.
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