The pesticide methoxychlor given orally during the perinatal/juvenile period, reduced the spermatogenic potential of males as adults by reducing their Sertoli cell number | Le pesticide methoxychlore donné oralement pendant la période périnatale et juvénile réduit le potentiel spermatogénique des mâles adultes en réduisant le nombre de cellules de Sertoli
2002
Johnson, L. ((Texas A and M University, College Station (Etats Unis). Center for Environmental and Rural Health, Department of Veterinary Anatomy and Public Health)) | Staub, C. | Silge, R.L. | Harris, M.W. | Chapin, R.E.
Perinatal and juvenile oral treatment of rats with the insecticide methoxychlor (MXC) reduced testicular size and other reproductive indices including the number of epididymal spermatozoa in those animals as adults. The objective was to determine if these males exposed during development had fewer Sertoli cells which might explain these testicular effects. Rat dams were gavaged with MXC at 0, 5, 50, or 150 mg/kg/day for the week before and after they gave birth. Resulting male pups (15/group) then were dosed directly from postnatal day 7 to 42. Testes were fixed in Bouin's and in OsO4, embedded in Epon and sectioned at 0.5 microm, stained with toluidine blue, and evaluated stereologically or cut at 20 microm to measure Sertoli cell nuclei with Nomarski optics. Sertoli cell number was calculated as the volume density of the nucleus times the parenchymal weight (90 per cent of testicular weight) divided by the volume of a single Sertoli cell nucleus. Across dose groups, there were no changes in the nuclear volume density, the volume of a single nucleus, or the number of Sertoli cells per g parenchyma. There were highly significant dose-related changes in the volume of Sertoli cell nuclei per testis and the number of Sertoli cells per testis. Reduced testicular weight (r = 0.94) and reduced numbers of epididymal spermatozoa (r = 0.43) were significantly (p 0.01) correlated to reduced number of Sertoli cells per testis. Hence, perinatal and juvenile oral exposure to MXC can reduce spermatogenic potential of males as adults by reducing their number of Sertoli cells
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