Development of female and male gametophytes in cereal species
2002
Timar, I. | Ponya, Z. | Szabo, L. | Barnabas, B.
The first steps in studies on the female and male gametophytes of wheat involved the light microscope analysis of semi-thin sections of embryo sacs containing egg-cells developing in planta. The information thus obtained on the successful isolation of egg-cell from its initial formation to maturity contributed to the succesful isolation of egg-cells. The morphological and ultrastructural details of egg-cells isolated 3, 6, 9, 12, 15 and 18 days after emasculation were examined to determine the function of the female gametophyte and its suitability for micromanipulation. A sufficient number of gametoplasts in the right stage of development are required for the succesful microinjection and in vitro fusion of egg-cells and male gametes.
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