Utilization of an indigenous marine luminous bacterium:Photobacterium leiognathi USTCMS 2116 as basis for the development of a biosensor for the rapid and inexpensive measurement of water toxicity
1998
Quinto,E.A. | Sevilla,F.III (University of Sto. Tomas,Espana, Manila (Philippines). Research Center for the Natural Sciences)
Thirty marine luminous bacterial strains were isolated from indigenous fishes, squids and from seawater in Manila Bay [Philippines]. A marine luminous bacterium isolated from squid and identified as Photobacterium leiognathi USTCMS 2116 exhibits very bright and stable luminescence as a result of its own metabolic activities when cultivated in Tryptore-Yeast-Extract-Glycerol-Seawater broth. It is very sensitive to chemical toxicants yielding a 49.5% decrease in luminance when exposed to the detergent sodium lauryl sulfate over a period of time. Freshly harvested chilled cell suspension of P. leiognathi USTCMS 2116 emits stable and high luminance readings for 8 minutes at room temperature. Exposure to minute amounts of common environmental toxicants like mercury, chloroform, copper, cyanide, zinc and phenol at concentrations in mg/L of 0.22, 0.60, 0.76, 4.28, 5.89, and 54.06, respectively for a period of 5 minutes inhibited the light output of the luminous cell suspension by 50%. Results indicate that the locally developed experimental toxicity assay based on bacterial bioluminescence utilizing a local strain:P. leiognathi USTCMS 2116 is a rapid and an inexpensive method of measuring water toxicity
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