Comparison of various methods for determination of antifungal activity of Bacillus coagulans
2002
Czaczyk, K. | Trojanowska, K. | Stachowiak, B. (Poznan University of Agriculture (Poland). Dept. of Biotechnology and Food Microbiology) | Zabielski, J.
The aim of this study was to compare various methods of antifungal activity determination in model experiments. For the investigations Bacillus coagulans (No. 6) isolate with the high antifungal activity, isolated from lupine composts was used. Antifungal activity of the examined isolate was estimated against five pathogenic species of fungi: Rhizoctonia solani, Bipolaris sorokiniana, Sclerotinia sclerotiorum, Trichothecium roseum and Fusarium oxysporum. Determination of fungal growth was carried out by three methods: ergosterol assessment, counting colony-forming units (CFU) and agar plate diffusion assay. Statistically significant Spearman's rank coefficients were recorded between ergosterol assessment and two other methods. On the basis of this data the assessment of ergosterol is the best way of determination of antifungal activity and may be used as a reference procedure
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