Plant regeneration from immature embryo cultures of Vigna unguiculata
2003
Choi, P.S. (Korea Research Inst. of Bioscience and Biotechnology, Tajeon (Korea). Eugentech Inc./Bioventure) | Cho, D.Y. | Soh, W.Y.
Mature and immature cotyledon explants of cowpea were cultured on Murashige and Skoog's (MS) medium supplemented with 0.1-2.0 mg/cubic dm benzyladenine (BA). Shoot organogenesis was observed from the minimal greenish calli formed at proximal cutting edges of the immature cotyledon explants after 15-20 d of culture. Among whole immature zygotic embryo and seven explant types we tested, single whole cotyledon was suitable for shoot organogenesis. Nearly 67.5 % of the explant types produced adventitious shoots on MS medium with 1 mg/cubic dm BA, and the shoot number (10.1) per explant was higher than other explant types. From the histological studies, the shoot primordia originated from the procambial strands of immature cotyledon explants. When the shoots were transferred to 1/2 MS basal medium, formed roots within 20 d of culture. The rooted plants were subsequently transferred to the pots and to the field.
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