Cross-resistance to strobilurin fungicides in mitochondrial and nuclear mutants of Saccharomyces cerevisiae
2003
Hnatova, M. | Gbelska, Y. | Obernauerova, M. | Subikova, V. | Subik, J. (Univerzita Komenskeho, Bratislava (Slovak Republic). Katedra Mikrobiologie a Virologie)
The resistance of Saccharomyces cerevisiae to three structurally different strobilurin fungicides, strobilurin A, kresoximethyl and azoxystrobin was investigated in this study. This resistance is under the control of both mitochondrial cob gene and the PDR network of nuclear genes involved in multidrug resistance. The mucidin-resistant muc1 and muc2 mutants of S. cerevisiae containing point mutations in mtDNA were found to be cross-resistant to kresoximethyl and azoxystrobin. Cross-resistance to all three strobilurin fungicides was also observed in yeast transformants containing gain-of-function mutations in the nuclear PDR3 gene. Nuclear mutants containing disrupted chromosomal copies of the PDR1 and PDR3 genes or the PDR5 gene alone were hypersensitive to kresoxim-methyl, azoxystrobin and strobilurin A. The results indicate that resistance to strobilurin fungicides, differing in chemical structure and specific activity, can be caused by the same molecular mechanism involving changes in the structure of apocytochrome b and/or increased efflux of strobilurins from fungal cells.
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