Activities of soluble and microsomal farnesyl diphosphatases in Datura stramonium
Ha, S.B. | Lee, D.E. | Lee, H.J. | Song, S.J. | Back, K. (Chonnam National Univ., Gwangju (South Korea). Dept. of Biotechnology)
The aim of this study was to investigate the activity of farnesyl diphosphatase (FDPase; EC 3.1.7.1) in Datura stramonium. The results show that FDPase is constitutively expressed at a high level in the soluble and the microsomal fractions of Datura stramonium. Soluble and microsomal FDPase have a similar pH optimum (5.0-6.0) and a similar substrate specificity. Geranyl diphosphate and geranylgeranyl diphosphate compete for FDP, but isopentenyl diphosphate, ATP, and para-nitrophenyl phosphate do not. Soluble FDPase activity was highest in fruit and flower followed by root, and leaf.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Library of Antonin Svehla