Large scale production and storability of encapsulated somatic embryos of mothbean (Vigna aconitifolia jacq)
Malabadi, R.B. | Nataraja, K.(Karnataka University, Dharwad (India). Division of Plant Tissue Culture)
Storability and germination of sodium alginate encapsulated somatic embryos of Vigna aconitifolia (Jacq.) cv. BMB-43 were tested on half strength Murashige and Skoog (MS) basal medium fortified with coconut water (10% v/v). The frequency of regeneration from encapsulatd embryos was affected significantly by concentration of sodium alginate and the duration of exposure to calcium chloride. Embryos encapsulated with 2.5% sodium alginate dissolved in MS basal salts solution recorded significantly higher germination than other treatments. A relatively short (5 min) incubation with calcium chloride solution provided uniform encapsulation of embryos that gave the highest percentage (65%) of germination. Synthetic seeds could be stored at 4øC for 50 days without reduction in viability as opposed to non - encapsulated somatic embryos which showed 6% viability after 20 days at 4øC. Germinated synthetic seeds produced normal plantlets.
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Эту запись предоставил Indian Council of Agricultural Research