Use of a Xanthine-Xanthine Oxidase System on in vitro Maturation and Fertilization in the Pig
2003
Sa, S.J. | Cheong, H.T. | Lee, S.Y. | Lee, J.H. | Ryu, I.S. | Yang, B.K. | Kim, J.I. | Park, C.K. (Kangwon University, Chunchon, Republic of Korea)
This study was undertake to evaluate the effects of catalse using xanthine(X)-xanthine oxidase system on in vitro maturation and fertilization in the pig. when follicular oocytes were cultured with X or XO, the maturation rates were not significantly different between in medium with and without catalse despite of different culture periods. However, significantly higher maturation rates were obtained in culture with X-XO-catlase system. The rates of degenerated oocutes were increased with culture periods prolonged, and were significantly higher in medium without that than with catalase at 120 h of culture. On the other, hand the parthenogenetic oocytes were observed with high proportions at 72h of culture, but were not different between the medium with and with out catalase at various times of culture, but were not different between the medium with and without catalase at various times of culture. in another experiment, the frozen-thawed boar spermatozoa treated with catalase at various times of culture.
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