Study on the Vitrification of Porcine GV and MII Oocytes after Removal of Cytoplasmic Lipid Droplets
2003
Choi, I.K. | Lee, S.J. | Song, H.B. (Daegue University, Daegue, Republic of Korea)
This study was conducted to investigated that the immature and mature oocytes of porcine can be cryopreserved by vitrification. Oocytes were centrifuged to polarize the cytoplasmic lipid droplets. The lipids were removed from cytoplasm by micromanipulation. Delipated oocytes were centrifuged after being preincubated with cytochalasin for 10 min, and lipid droplets were removed. Centrifuged oocytes were treated with CB and centriguged to polorize lipid dropets but not delipated and control oocytes is not-treatment. Oocytes of three types were vitrified in electron microscope grids
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