Embryogenic calli formation and plant regeneration responses in yellow and white maize (Zea mays L.) inbred lines
2003
Damasco, O.P. | Avenido, R.A. | Caymo, L.S. | Sotelo, SJ.P. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Genetic engineering (transgenic) technology offers a new tool in developing improved maize varieties. Identification of inbred lines with high embryonic and regeneration potential is an important requirement for genetic manipulation of maize. Forty nine yellow and white maize inbred lines developed by the Institute of Plant Breeding (IPB), UPLB [Philippines Univ. Los Banos, College, Laguna, Philippines] and International Maize and Wheat Improvement Center (CIMMYT), Mexico were evaluated for Embryonic calli formation and shoot/plant regeneration. Immature embryos (10-12 days after pollination) were cultured onto two callus induction media (NGC1); Bohorova et al., 1997 and CM3; Perez and Damasco, 1997) incubated in the dark for 6-8 weeks and generated under 16h photoperiod. The percent E-calli formation from 49 inbred lines evaluated ranged from 0-96%, with only 32 inbred lines producing E-calli. Two types of E-calli were produced: Type 1 (compact, nodular with many scutellar like bodies) and Type 2 (friable, creamy color). Shoot regeneration from E-calli was observed in 22 inbred lines and ranged from 6.2%-100%. Except for IPB inbred line Var 4, the conversion of regenerated shoots into whole plant was relatively low, majority of the lines evaluated showed less than 50% whole plant regeneration. Survival of the tissue cultured maize ex vitro ranged from 33.3%-92% and 6.7%-100% upon establishment. On the basis of E-calli formation and shoot/plant regeneration, five IPB inbred lines, Var 4, Var 2 and P 53 (white) and Pi 17 and Pi 23 (yellow) and two CIMMYT inbred lines, CL00331 and ARMMP 36, were selected as suitable lines for transformation
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