Study of the external and internal morphology of Pisum sativum L., with growth hormones i.e., indole-3-acetic acid and kinetin and heavy metal i.e., lead nitrate
2003
Chaudhry, N.Y. | Rasheed, S. (Punjab Univ., Lahore (Pakistan). Dept. of Botany)
The morphological features of root and shoot of Pisum sativum L., were examined after 30 days. The plant was treated with growth hormones i.e., IAA and Kinetin and heavy metal i.e., Pb (NO3)2. Hormones and heavy metal were used individually i.e., 50 ppm IAA, 50 ppm Kinetin and 50 ppm Pb and in combinations i.e., 50 ppm IAA + 50 ppm Kinetin, 50 ppm IAA + 50 ppm Pb, 50 ppm Kinetin + 50 ppm Pb and 50 ppm IAA + 50 ppm Kinetin + 50 ppm Pb. With applied IAA inhibition in length was observed which was followed by an increase in the diameter in both root and shoot. Extension growth was observed with Kinetin, however, Pb showed inhibition. The number of lateral roots showed increase with IAA and Kinetin but inhibition was observed with Pb. In the mixed doses, length was inhibited. This inhibition was accompanied by expansion in diameter of root and shoot. Moreover, lateral roots showed increase. In the internal morphology of root, an increase in the number of metaxylem elements as well as in the width of these elements was observed with all treatments except Pb. In shoot, the increase in the width of epidermal cells as well as in the cortical region, the number of metaxylem elements, width of these elements and the width of pith region responded positively with all treatments except Pb. Application of heavy metal i.e., Pb(NO3)2 revealed inhibitory effects however, growth hormones i.e., IAA and Kinetin not only balanced the disturbed growth caused by the heavy metal but enhanced the root and stem growth to a certain extent.
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Эту запись предоставил National Agricultural Research Centre