Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone
2003
Cajza, M. | Folkman, W. (Institute of Plant Protection, Poznan (Poland))
In performed experiments, insoluble polyvinylpolypyrrolidone, PVPP as an additive to the extraction buffer was used for isolation of total nucleic acids from hop plants and grapevine in order to obtain templates useful for detection of HLVd and HSVd by means of RT-PCR. Addition of 2 percent of PVPP to the original GTC buffer (Chomczynski and Sacchi, 1987) appeared to be the most favourable. Due to PVPP addition, the protocol of extraction of nucleic acids was simplified by shortening of isolation time and reduction of expenses. However, application of the simplified method for obtaining of templates that guaranteed full repeatability of test results was limited to the spring and early summer season
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