Detecting meat-and-bone meal in ruminant's feeds by species-specific PCR
2003
Cheng, Y.H. (National I-Lan University, I-Lan (Taiwan). Dept. of Animal Science) | Wen, C.M. (National Kao-Hsiung, Kao-Hsiung (Taiwan). Dept. of Life Science) | Ding, S.T. (National Taiwan University, Taipei (Taiwan). Dept. of Animal Science) | Kao, C.C. | Kuo, T.Y. (National I-Lan University, I-Lan (Taiwan). Dept. of Animal Science)
Bovine spongiform encephalopathy may have arisen from feeding scrapie infected meat-and-bone meal (MBM) to cattle that was produced under sub-optimal conditions. The study utilized a polymerase chain reaction (PCR) assay to identify bovine, porcine, ovine, and chicken MBM in animal diets. In order to confirm the specific amplification of expected products from MBM for each species, the PCR products were digested with restriction enzymes Hphl, MnlI, Sspl and HindIII and different length polymorphisms were observed. In the sensivity assays, concentration as low as 1 percent of MBM in animal feeds could be detected by PCR procedure. Based on the prevalence survey for commercial MBM in Taiwan, it predominantly consisted of bovine porcine origins. This molecular approach provides a quick, highly reproducible and sensitive method to detect the MBM in feeds
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