Antibody production against hormones for radioimmunoassay I. Comparison among chicken, rabbit and guinea pig in antibody production against progesterone
1992
Petai Pongpiachan | Puntipa Pongpiachan (Chiang Mai Univ., Chiang Mai (Thailand). Faculty of Agriculture. Dept. of Animal Husbandry)
Current experiment aim to raise antisera against progesterone in chicken, rabbit and Guinea pig by 11 Alpha-Hydroxyprogesterone-hemisuccinate-Bovine Serum Albumin to which self-prepared (Conjugate P) and external source (Conjugate S) were also assessed. The experiment were conducted at Department of Animal Husbandry, Faculty of Agriculture, Chiang Mai University. Nine each of ISA Brown layer, Native x New Zealand White rabbit and Guinea pigs were used, Within each species, 3 animals per group were immunized with Freund's Complete Adjuvant (FCA) only, FCA + 20 microgram Conjugate P, and FCA + 20 microgram Conjugate S by subcutaneous injection at 3 points along the mid-line of the back. Number of immunization were 4 times at 2 weeeks interval. The result showed that rabbits were capable of producing the antibody better than chicken and Guinea pig. Rabbit and chicken produced greater amount of antibody when immunized with Conjugate P in comparison with Conjugate S while Guinea pig responded better to Conjugate S. However, there was individual variation within species in the antibody production. The achievement of the present experiment are as : (1) availability of antibody to progesterone for further radioimmunoassay (2) conjugation technique between steriod and protein for future active immunization experiment.
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Эту запись предоставил Kasetsart University