Optimization of chemical biochemical and physical treatments for baker's yeast lysis for yeast extract production
1998
Prasart Foo-trakul | Supot Boonraeng | Weerasit Kanlayakrit | Charan Chetanachitra (Kasetsart Univ., Bangkok (Thailand). Faculty of Agro-Industry. Dept. of Biotechnology)
Effects of chemical, biochemical and physical treatments on baker's yeast lysis were studied. The standard conditions for autolysis were pH 5.2, temperature 54 deg C and yeast concentration 13 percent dry wt. The optimum chemical treatment obtained by using 5 percent v/v ethanol and 5 percent w/v NaCl. The optimum biochemical and physical treatments were the use of 0.1 percent w/w of papain and 8,000 psi of homogenization respectively. The combination treatment of 5 percent ethanol and 5 percent NaCl exhibited highest protein content (61.90 percent). The physical treated sample obtained 42.45 percent of total carbohydrate yield and biochemical treated sample obtained 46.53 percent of autolysate. The chemical analysis of the samples showed the best result in the biochemical treated sample as (g/100g dry wt): 57.50 of total protein, 8.22 of amino-nitrogen, 24.27 of carbohydrate, 7.86 of nucleic acid and 10.72 of ash contents. This treatment showed the feasibility of producing yeast extract, yeast autolysate at pilot plant or industrial scale.
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Эту запись предоставил Kasetsart University