Recent potential in the biotechnology of mangosteen II: Cultivar improvement
1998
Sompong Te-chato (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
Somaclonal variation obtained from culturing apomict seed of mangosteen on modified Murashige and Skoog (MMS) medium supplemented with 25 micro M benzyladenine (BA) for 8 weeks consisted three types of phenotypic variants; chimera, malformed leaves and chlorosis. Phenotypic variation frequency of those variants ranged from 6*10*[-4) (for chimera) to 3*10*[-2) (for chlorosis). In the case of induced mutation, gamma irradiation at 10 grays and 0.5 percent ethylmethane sulfonate (EMS) caused more than 50 percent decrement of percent leaf forming callus. DNA analyses of calli induced from treated and non-treated leaves by randomly amplified polymorphic technique (RAPD) revealed difference polymorphisms. This confirms radio- and chemo-sensitivity of the two different types of mutagens. The results of DNA analyses were ralated with an inhibitory effect on percent leaf forming callus.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Kasetsart University