Immunostimulant and vaccination in black tiger shrimp, Penaeus monodon Fabricius: I. Extraction of beta-glucan from yeast and its application in black tiger shrimp (Penaeus monodon Fabricius)
2000
Sitthipun, M. | Hanpongkittikul, A. | Supamattaya, K. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Agro-Industry. Dept. of Industrial Biotechnology)
Saccharomyces cerevisiae TISTR 5088 cultivated at room temperature in the shaker (200 rpm) for 36 hours in YEPD medium had 9.0 g/l cell dry weight. After cell homogenization and extraction the yield from the cell wall was 13.65 percent of cell dry weight. The beta-glucan was extracted from the cell wall by 2 methods: (1) extraction with 50 mM phosphate buffer (pH 8.0 at 9.0 deg C) provided crude bete-glucan 20.0 percent of cell wall with total sugar 129.0 mg/g and (2) extraction with 0.75 M sodium hydroxide at 75 deg C provided crude beta-glucan 30.0 percent of cell wall with total sugar 82.2 mg/g. Purificantion of the 0.02 g/ml beta-glucan solution by Sephadex G-100 column (1.0*110 cm) contained 2.85 mg/ml total sugar. Separation by thin layer chromatography showed that samples had the same Rf as the standard beta-glucan. Four test diets were formulated, 8.4 g of yeast cell wall/kg feed, crude beta-glucan 1.70 g/kg feed, 1.0 g of pure beta-glucan/kg feed and control feed. The shrimp fed with pure beta-glucan diet showed the highest number of total haemocyte count of 9.90+-1.52*10*[4) cell/ml and exhibited better clearance ability after injection with Vibrio harveyi as well as higher disease resistance against V. harveyi (90.0 percent) when compare to shrimp fed with other test diet.
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Эту запись предоставил Kasetsart University