Inhibition of Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails
Laukova, A.(Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat)E-mail:[email protected] | Guba, P.(Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic)) | Nemcova, R.(Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic)) | Marekova, M.(Univerzita Pavla Jozefa Safarika, Kosice (Slovak Republic). Ustav Lekarskej Chemie, Biochemie a Klinickej Biochemie)
The protective (ENT1) and therapeutic (ENT2) effects of enterocin A (Ent), produced by Enterococcus faecium EK13, against Salmonella enterica serovar Dusseldorf SA31 was determined in a model of gnotobiotic Japanese quails. Twenty-one 3 days old birds were divided into 3 groups of equal size. They were experimentally infected with SA31 (10,000,000 cfu/mL) per os and treated as follows: 1) Ent A (200 microL of 25,600 AU/mL) was administered 8 h before infection with SA31 strain (group ENT1), 2) Ent A was administered 8 h after infection (group ENT2, 3) control group CG. Sampling of the feces was performed 8, 24, 48 and 168 h after infection and the content of intestines was analyzed at the end of the experiment. A log 1.37 reduction of SA31 colonization in feces of the group ENT1 was found after 8 h in comparison with CG. After 24 h, a significant difference was observed when comparing CG and ENT2. After 48 h, a lower colonization of SA31 was found in both groups which continued until 168 h. At the same time, reduction of Salmonella enterica serovar Dusseldorf was detected in the content of the caecum (2.44 log) and ileum (3.16 log) in ENT2 but not in ENT 1 when compared with control group. These observations indicate stronger therapeutic than prophylactic effect of Ent A in the digestive tract of gnotobiotic Japanese quails.
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