Effect of extenders and thaw temperature on the acrosomal integrity of ram spermatozoa

Mathur, A.K.

Effect of extenders and thaw temperature on the acrosomal integrity of ram spermatozoa

2003

Mathur, A.K. (Project Directorate on Cattle, Merrut Cantt (India))

Pooled ejaculates of Rambouillet ram semen was extended in egg yolk-raffinose-citrate-glutamic acid-glycerol (EYRCGG),egg yolk lactose-raffinose-citrate-glycerol (EYLRCG)and egg yolk tris glucose (EYTG)extenders @ 1x10 (9) spermatozoa/ml and subjected to equilibration followed by freezing into pellets of 0.2 ml size. Smears were made at extension, post-equilibration and at thawing which were done at 37, 50 and 60§C in a dry test tube. Staioning was done by Giemsa stain and morphology was evaluated. The acrosomal integrity was not affected significantly by diluents at post dilution as well as post equilibration stages. At post-thaw stage, irrespective of thawing regimen, acrosomal integrity of frozen-thawed ram spertmatozoa was not influenced by the extenders, however, thaw temperature had a significant effect (P0.01) on acrosomal integrity with higher values at 50§C. The over all means were 54.9ñ0.43, 56.6ñ0.32 and 55.0ñ 0.38% at thaw temperatures of 37, 50 and 60§C respectively.

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