Effect of sex on osteoclast formation in chicken bone marrow cell cultures
2001
Kikuchi, N. (Niigata Univ. (Japan). Faculty of Agriculture) | Sugiyama, T. | Kusuhara, S.
In the present study, in order to examine the effect of sex on the osteoclast formation in bone marrow, we cultured bone marrow cells isolated from egg-laying hens and male adult chickens, and observed the behaviors of these cells with tartrate-resistant acid phosphatase (TRAP) activity and hematoxylin staining. As a result, TRAP-positive mononuclear cells, osteoclast precursors and TRAP-positive multinuclear cells, osteoclast-like cells were observed in the culturing. Especially, in the culture of bone marrow cells isolated from the hen medullary bone, many precursors and osteoclast-like cells were formed. Also, there are many TRAP-negative fibroblast-like cells such as stromal cells or osteoblasts. On the other hand, in the culture of bone marrow cells isolated from male chicken, the number of precursor was only 20% of that isolated from egg-laying hen, and few osteoclast-like cells were observed. These results suggested that there are many precursors in hen medullary bone marrow cells, and then the osteoclast formation frequently occurs, while, there are a few precursors in the bone marrow cells of adult male chicken, and few osteoclasts form.
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