Immunohistochemical quantification of fast-myosin in upper hind limb muscles of the pig
2001
Manabe, N. (Kyoto Univ. (Japan)) | Irie, M. | Mitsumoto, M. | Furuya, Y. | Yamaguchi, M. | Miyamoto, H.
Using an immunohistochemical microquantification technique, in which frozen sections mounted on glass slides are used as analogues of the antigen precoated wells of the enzyme-linked immunosorbent assay-plates and the structure is well preserved, we examined the contents of fastmyosin isoforms in different portions of 17 hind limb muscles of female Duroc pigs. High fast-myosin levels (more than 200mg/g of total protein) were observed in the glutens superficialis, glutens medius, vastus lateralis, bicepsfemoris and tensorfasciae latae muscles, and low levels (less than 75mg/g) in the glutens profundus, vastus intermedius and semimembmnosus muscles. Fast-myosin-positive fibers were distributed more in the superficial regions than in the deeper regions in the hind limb muscles. The highest population of type 1 fibers was seen in the vastus intermedius muscle (78%), and the lowest in the gluteus medius muscle (10%). The highest number of type 2A fibers was seen in the rectus femoris muscle (29%), while the lowest was seen in the semimembmnosus muscle (14%). The highest number of type 2B fibers was found in the gluteus medius muscle (73%), and the lowest in the vastus intermedius muscle (7%). Good correlation between the microquantification and histochemical analysis was seen. The histological structure of the muscles was well preserved during microquantification, and this technique could be used in research projects to study the contractile properties of the muscles and evaluate the meat qualities of pig muscles.
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