Epitope-mapping of antigen-specific T lymphocyte in cattle immunized with recombinant major piroplasm surface protein of Theileria sergenti
2001
Kakuda, T. (Kitasato Univ., Towada, Aomori (Japan). Coll. of Veterinary and Animal Sciences) | Sugimoto, C. | Onuma, M.
The cellular immune responses against major piroplasm surface protein (MPSP) of Theileria sergenti were characterized. Three cattle were immunized with recombinant MPSP (C type) encapsulated by mannan-coated liposome. The proliferative responses of peripheral blood mononuclear cells (PBMC) against MPSP were detected in all immunized animals. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that T celi lines derived from each animal expressed relatively high levels of interferon (IFN)-gamma mRNA, low levels of interleukin (IL)-2. IL- 10, and tumor necrosis factor (TNF)-alpha mRNAs, but no detectable level of IL-4 mRNA. From the results of T cell epitope-mapping, T-cell lines from two animais responded to DTSKFTPTVAHRLKHAEDLF (residues 61 to 80), while other animal responded to GTGKVYDFVGNFKVTKVKFE (residues 141 to 160). The MPSP-type specific response of a T-cell line was observed in the latter region of MPSP. These data suggest that immunization with cocktail vaccine consisting of different types of MPSP may be necessary in the field trial.
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