Redox changes accompanying the degradation of seed storage proteins in germinating rice [Oryza sativa]

Yano, H.; Wong, J.H.; Cho, M.J.; Buchanan, B.B.

Redox changes accompanying the degradation of seed storage proteins in germinating rice [Oryza sativa]

2001

Yano, H. (University of California, Berkeley (USA)) | Wong, J.H. | Cho, M.J. | Buchanan, B.B.

The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.

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